Abstract
Microbiological Aspects in Biofilm Produced by some Uropathogens Isolated from Patients with Indwelling Bladder Catheters
Highlights
Out of 75 catheter urine specimens, 61 (81.4%) were positive for culture including: Escherichia coli 17 (27.9%) followed by Klebsiella spp. 13 (21.3%), Pseudomonas aeruginosa 12 (19.7%), and in spectrophotometric method, the results showed that all of 12 isolates of Pseudomonas aeruginosa, 7 isolates of Proteus mirabilis, 17 isolates of Escherichia coli and 13 isolates of Klebsiella, 12 (100%), 7 (100%), 12 (70.6%) and 12 (92.3%) isolates produced biofilm in the glucose supplemented media
Among 61 (81.4%) culture positive cases, the yielded microbial causative agents were distributed as the followings: Escherichia coli 17 (27.9%), Klebsiella spp. 13 (21.3%), Pseudomonas aeruginosa 12 (19.7%), Staphylococci 9 (14.8%) divided into 4 (44.4%) Staphylococcus aureus and 5 (55.6%) Staphylococcus epidermidis, Proteus mirabilis 7 (11.5%), Enterobacter spp. 1 (1.6), in addition to Candida spp which was found as a mixed form with the above mentioned bacteria in 2 (3.3%) cases
Under the field of biofilm production, qualitative biofilm assay, tube method, our result showed that out of 17 (27.9 %) isolates of Escherichia coli, 13 (21.3 %) isolates of Klebsiella species, 12 (19.7 %) isolates of Pseudomonas aeruginosa, and 7 (11.5 %) Proteus mirabilis, biofilm were produced on the inner lining of the tubes in (64.7 %), 9 (69.2 %), (100 %), and 7 (100 %) respectively
Summary
Out of 75 catheter urine specimen, 61 (81.4%) were culture positive, 7 (9.3%) sterile urine while the other 7 (9.3%) were defined as sterile Pyuria. Under the field of biofilm production, qualitative biofilm assay, tube method, our result showed that out of 17 (27.9 %) isolates of Escherichia coli, 13 (21.3 %) isolates of Klebsiella species, 12 (19.7 %) isolates of Pseudomonas aeruginosa, and 7 (11.5 %) Proteus mirabilis, biofilm were produced on the inner lining of the tubes in (64.7 %), 9 (69.2 %), (100 %), and 7 (100 %) respectively. In the other qualitative assay (foley catheter method) our study revealed that out of 17 isolates of Escherichia coli, 13 isolates of Klebsiella species, 12 isolates of Pseudomomnas aeruginosa, and 7 Proteus mirabilis, biofilm were produced on the surface of the catheters in 11 (64.7%), (84.6 %), (100%), 7 (100%) respectively.
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