MICROBIOLOGICAL AND SANITARY - CHEMICAL CHARACTERISTICS OF WASTEWATER FROM PHARMACEUTICAL COMPANY

Abstract

Aim. Оssessment of wastewater from a pharmaceutical company according to sanitary-microbiological and chemical indicators to determine the method of treatment and remediation of them from pollutants and pathogens. Methods. In sanitary and bacteriological studies, classical microbiological methods were used to determine the total microbial number of pathogenic bacteria. Determination of the concentration of ammonium, nitrite and nitrate in wastewater samples from a pharmaceutical company was carried out spectrophotometrically using the chemical reaction of ions to Nessler's reagent, Griss reagent, and phenol sulfide acid. The content of heavy metal ions was determined by the method of electrothermal AAS using the device "Saturn-2", by the photometric method using 4-aminoantipyrine – phenol, by the extraction-photometric method – by surfactants, by the method of infrared spectrometry – by petroleum hydrocarbons. Results. It was experimentally confirmed that the total microbial count in wastewater samples from a pharmaceutical company slightly exceeded the standard value. The BGKP index and the E. coli index were within the normal range. The pathogenic bacteria Salmonella moscow and Klebsiella pneumoniae were found in the wastewater of pharmaceutical company. The contamination of pharmaceutical stock can be judged by the 8.5 times excess of the concentration of ammonium ions in them compared to the MPC. The results of chemical analysis of wastewater samples from a pharmaceutical company indicate their multicomponent composition.Of the heavy metal ions, ions of zinc, copper, lead, hexavalent chromium and cadmium were mainly detected. With the exception of cadmium ions, the concentration of heavy metal ions was 1.4–7.2 times higher than the MPC norm. Anionic surfactants predominated in pharmaceutical stocks – their concentration was 8.2 ± 0.7 mg/dm3. The level of phenolic and oil pollution of wastewater was minimal in comparison with the content of anionic surfactants, which could be caused by the activity of aboriginal strains of bacteria-destructors with a high phenolic capacity. Conclusion. The results of sanitary-microbiological and chemical analysis of wastewaters of a pharmaceutical company indicate their multicomponent nature and environmental hazard – they contain pathogenic bacteria Salmonella moscow and Klebsiella pneumoniae and pollutants of various nature: heavy metal ions Cu (II), Cr (VI) and Pb (II) at a concentration of 36.0 ± 14 μg/dm3, 3.0 ± 0.4 μg/dm3 and 14.0 ± 0.7 μg/dm3, respectively, and organic pollutants – phenol, oil products and anionic surfactants at a concentration of 0.003 ± 0.0006 mg/dm3, 0.81 ± 0.05 mg/dm3 and 8.2 ± 0.7 mg/dm3, respectively. Werecommend to carry out a comprehensive purification of wastewater from a pharmaceutical company from phenol, heavy metal ions [Cu (II), Cr (VI), Zn (II)], oil products, anionic surfactants and pathogens.