Microbicidity of blood neutrophils in pigs at different schemes of use of the feed additive «Protamin»

Abstract

Purpose. to study the influence of the feeding scheme of the feed additive «Yeast hydrolyzate “Protamine”» on the activity of microbicidal mechanisms of blood neutrophils in pigs.Materials and methods. In the conditions of the Baikovo pig farm in the Bryansk region, 3 groups of 10 piglets of 58-61 days of age (on growing) with a live weight of 11.16 ± 0.01 kg were formed from sows (large white x Landrace), inseminated with Duroc boar sperm. Animals of group 1 were control, piglets of group 2 received 5 grams of feed supplement «Yeast hydrolyzate “Protamin”» with water once a day for 75 days according to the scheme: 7 days — watering, 7 days — break; animals of the 3rd group also received once a day with water 5 grams of feed additive «Yeast hydrolyzate “Protamin“» for 75 days, but according to the scheme: 7 days — drinking, 14 days — a break. Blood for the study was taken from 5 animals of each group from the jugular vein in the morning before feeding before the start of the experiment, after 35 and 75 days, and also one month after the end of drinking the drug. One month before the 1st blood sampling, all piglets were vaccinated against classical swine fever. One month before the 2nd blood sampling, all piglets were vaccinated against swine erysipelas, and the day before this blood sampling, they were revaccinated against classical swine fever. One month before 3 blood sampling, all pigs were revaccinated against swine erysipelas. A month after the end of the feeding of the feed additive to the experimental animals, pigs of all groups were subjected to technological stress — they began to prepare for slaughter: they began to starve, carried out a veterinary examination and weighing, after which they took blood for analysis. It was found that post-vaccination stress caused a more pronounced decrease in oxygen-dependent and oxygen-independent microbicidal activity of blood neutrophils than pre-slaughter stress.Results. The use of the feed additive «Protamin» according to both schemes contributed to an increase in oxygen-independent microbicidal activity and the appearance of an adaptation reserve of oxygen-dependent microbicidal activity, which is more pronounced in pigs when using the drug according to the scheme: 7 days — drinking, 7 days — a break. The absence of stressful influences led to the optimization of homeostasis.