Microbially mediated arsenic mobilization in the clay layer and underlying aquifer in the Hetao Basin, Inner Mongolia, China.

Abstract

The clay layer is a source to facilitate arsenic (As) enrichment in the aquifer. However, little is known about microbial processes in the clay layer and their roles in As mobilization in the underlying aquifer. In this study, high-throughput sequencing of full-length 16S rRNA gene and metagenomics were used to characterize the microbial composition and functional potential in a sediment borehole across the clay and sand layers in Hetao Basin, Inner Mongolia, China. Results showed the significant differences between the clay layer and underlying sand layer in the geochemistry, microbial composition and functional potential. Fermentation, Fe(III) reduction, As(V) reduction, sulfate reduction, thiosulfate disproportionation, reductive tricarboxylic acid and Wood-Ljungdahl pathway identified in sediments from the clay layer were positively correlated to the observed high levels of TOC, soluble ammonium, H3PO4-extractable As(III) and As(V) and HCl-extractable Fe(II). Although the microbial compositions of the clay and sand layers were different, the microbial functional potential at the interface between the clay and sand layers was similar with the characteristics of fermentation, ammonification and As(V) reduction. The similarity of microbial functional potential at the interface may be attributable to the interaction between the sand and adjacent clay layer with the fluctuation of groundwater level. These metabolic products from the microbial processes in the clay layers and interface would migrate into the underlying groundwater during groundwater overpumping, which facilitates As enrichment in groundwater.