Microbial xylanases and their inhibition by specific proteins in cereals

Daniela Chmelová,Dominika Škulcová,Miroslav Ondrejovic

doi:10.18832/kp2019.65.127

Daniela Chmelová, Dominika Škulcová + Show 1 more

Open Access

https://doi.org/10.18832/kp2019.65.127

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Abstract

Arabinoxylans (AXs) belong to the components of plant cells which are mainly degraded by microbial xylanases during colonization of grain by phytopathogens. For the defence, cereals contain proteinaceous xylanase inhibitors (XIs), namely xylanase inhibitor protein (XIP), Triticum aestivum xylanase inhibitor (TAXI) and thaumatin-like xylanase inhibitor (TLXI). Their presence in cereals in high levels can be a serious problem in different industrial applications. XIs regulate AX hydrolysis and participate in plant defence mechanisms. XIs have various specificity against microbial xylanases from the glycoside hydrolase (GH) families of GH10 and GH11. Therefore, this review brings new information about the function of XIs as defence responses to pathogen infection of plants and as a problem in plant material processing in different industrial applications.

Highlights

Arabinoxylans (AXs) and glucans are non-starch polysaccharides present in cereal grains and these cell wall components were confirmed in barley (Egi et al, 2004)
Insufficient quality of cereal grains has a negative impact on consumer health
It seems that xylanase inhibitors (XIs) can be a suitable tool for cereal grain defence to pathogenesis and accumulation of mycotoxins

Summary

Introduction

Arabinoxylans (AXs) and glucans are non-starch polysaccharides present in cereal grains and these cell wall components were confirmed in barley (Egi et al, 2004). For the regulation of their activity, cereals produce their own xylanases in the form of the xylanase precursor in the aleurone cells (Caspers et al, 2001; Simpson et al, 2003; Selvaraj et al, 2010) and xylanase inhibitors (XIs) controlling the AX hydrolysis and the utilization of starchy resources (Caspers et al, 2001; Evers and Millar, 2002) These processes play the key role in the natural defence to disease (Payan et al, 2004; Dornez et al, 2010; Zhan et al, 2017a; Sun et al, 2018). The aim of this study was to describe the function of XIs against xylanases of the GH10 and GH11 families and defence responses to pathogen infection of plants

Xylanase inhibitors

Findings

Conclusion