Abstract
Black liquor is released from pulp and paper industry. It is hazardous in nature and harmful to environment. We have screened bacterial cultures for treatment of black liquor. 18 bacterial cultures were used for screening of biodegradation of black liquor (1:5 diluted). We have used two different pH i.e. 9.0 and 5.5 for study. Among bacterial cultures we attempted to use Pseudomonas sp., Arthrobacter, Pseudomonas fluroscence and Pseudomonas pudita for screening. Baker's yeast was also used for study at pH 9.0 and 5.5. Study of sugar utilization, phenol degradation and decolorization was carried out. Pseudomonas fluorescens NCIM 2174 utilized 47.5% sugar at pH 9. In case phenol degradation, 30.35% degradation was observed by Arthrobacter simplex NCIM 2449 at pH 9. 27.85% decolorization by done by Pseudomonas putida NCIM 2872 at pH 5.5. Pseudomonas fluorescens NCIM 2174 has shown 10.36% phenol degradation and 2.76% decolorization at pH 9. Arthrobacter simplex NCIM 2449 utilized 37.7% sugar and 8.23% decolorization observed at pH 9. 4.34% sugar utilization and 17.71% phenol degradation was observed with Pseudomonas putida NCIM 2872 at pH 5.5. Baker's yeast has shown 10.94% sugar utilization and 15.59% decolorization at pH 5.5. 14.27% phenol degradaton was obseved with Baker's yeast at pH 9.
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