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Abstract
Abstract Background Biotransformation of quinic acid to shikimic acid was attempted using whole cells of Bacillus megaterium as a biocatalyst. Results Physico-chemical parameters such as temperature (37°C), pH (7.0), agitation (200 rpm), substrate (5 mM) and cell mass concentrations (200 kg/m 3) and reaction time (3 h) were found optimum to enhance the bioconversion. Maximum conversion (89%) of quinic acid to shikimic acid was achieved using the above optimized parameters. Shikimic acid was extracted from the reaction mixture by a pH-dependent method and maximum recovery (76%) was obtained with petroleum ether. Conclusions Biotransformation of quinic acid to shikimic acid seems to be a better alternative over its fermentative production.
Highlights
Biotransformation of quinic acid to shikimic acid was attempted using whole cells of Bacillus megaterium as a biocatalyst
It is estimated that nearly twothirds of the requirement of shikimic acid is still being sourced from plants, with the remaining one-third only obtained from genetically engineered Escherichia coli [8,9]
Whole cells of Bacillus megaterium microbial type culture collection (MTCC) 428 were used as biocatalyst for the transformation of quinic acid to shikimic acid
Summary
Biotransformation of quinic acid to shikimic acid was attempted using whole cells of Bacillus megaterium as a biocatalyst. A key intermediate of the aromatic amino acid synthesis pathway [1] has immense pharmaceutical importance. This pathway is present in animals, plants and even in microorganisms as the common route for the synthesis of aromatic compounds [2,3,4]. Whole cells of Bacillus megaterium MTCC 428 were used as biocatalyst for the transformation of quinic acid to shikimic acid. Various physico-chemical parameters of the biochemical reaction were optimized to enhance the yield of shikimic acid
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