Abstract
Microorganisms have been screened for biotransformational activity against diosgenin or its precursor furostanol glycosides obtained from fenugreek seed ( Trigonella foenumgraecum). Activities against the genin and precursor glycoside substrates were unrelated. Using diosgenin as the substrate, five strains produced diosgenone and some 1-dehydrodisogenone, whereas Mycobacterium phlei produced some androstenes when α,α′-bipyridyl was added. Fusarium solani produced diosgenin in > 80% yield from the glycoside, thus eliminating the need for acid hydrolysis prior to modification of the steroid ring. The transformation of diosgenin to diosgenone by Nocardia rhodochrous was optimized and found to be mediated by a cholesterol oxidase. This enzyme retained its activity after extraction from the cell and also when used in a CCl 4/water emulsion.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
