Abstract
Copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxypropionate (3HP) were synthesized in Alcaligenes latus from mixed carbon substrates of sucrose and 3-hydroxypropionic acid. The composition of copolyesters varied from 0 to 26 mol% 3HP, depending on the fraction of carbon substrates. The microbial copolyester was characterized by 1H and 13C nuclear magnetic resonance (n.m.r.) spectroscopy, gel permeation chromatography and differential scanning calorimetry. The copolymers were shown to have a random sequence distribution of 3HB and 3HP units by analysis of the 125 MHz 13C n.m.r. spectra. The biosynthetic pathway of copolymers has been discussed.
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