Microbial stoichiometric flexibility regulates rice straw mineralization and its priming effect in paddy soil

Abstract

Nitrogen (N) and phosphorus (P) availability plays a crucial role in carbon (C) cycling in terrestrial ecosystems. However, the C:N:P stoichiometric regulation of microbial mineralization of plant residues and its impact on the soil priming effect (PE), measured as CO2 and CH4 emission, in paddy soils remain unclear. In this study, the effect of soil C:N:P stoichiometry (regulated by the application of N and P fertilizers) on the mineralization of 13C-labelled rice straw and the subsequent PE was investigated in a 100-day incubation experiment in flooded paddy soil. N and P additions increased straw mineralization by approximately 25% and 10%, respectively. Additions of both N and P led to higher CO2 efflux, but lower CH4 emission. With an increase in the ratios of DOC:NH4+-N, DOC:Olsen P, and microbial biomass C:N, 13CO2 efflux increased exponentially to a maximum. Compared with sole straw addition, exclusive N addition led to a weaker PE for CO2 emission, whereas exclusive P addition induced a stronger PE for CO2 emission. In contrast, CH4 emitted from native soil organic matter (SOM) was reduced by 7.4% and 46.1% following P and NP application, respectively. Structural equation models suggest that available N had dominant and direct positive effects, whereas microbial biomass stoichiometry mainly exerted negative indirect effects on PE. The stoichiometry of soil enzyme activity directly down-regulated CH4 emission from SOM. Microbes obviously regulate soil C turnover via stoichiometric flexibility to maintain an elemental stoichiometric balance between resources and microbial requirements. The addition of straw in combination with N and P fertilization in paddy soils could therefore meet microbial stoichiometric requirements and regulate microbial activity and extracellular enzyme production, resulting in co-metabolism of fresh C and native SOM.