Microbial Stereo Inversion of (R) 3 Chloro-1,2-Propandiol by Wickerhamomyces anomalous MGR6-KY209903

Abstract

Our interest has been focused on the production of chiral compounds by the method of enantioselective microbial transformations of prochiral starting material with yeast. The preliminary assimilation was exercised with Wickerhamomyces anomalous MGR6 grown on synthetic medium and observed assimilation by pH change of the medium.The specific rotation of (R-3- Chloro-1,2-propanediol) was -5 (c=2.5, in C2H5OH) in conditions with enatiomeric excess of 85.6 % Wickerhamomyces anomalous product. The structural and dynamic properties of R-3-Chloro-1,2-propanediol in GCMS spectrum, the most prevailing compound among them retention time of 9.63 was obtained. The IR Spectrum, a shallow broad band at wavelength 3290.56 signifies the presence of O-H stretching, the narrow peak both at 1637.56 indicates the presence of C=O stretching in conjugation with functional group of R- 3 Chloro-1,2-propanediol(C3H7ClO2).The sharp peaks in HPLC spectrum of R 3 Chloro-1,2-propanediol in the retention time peak were 2.47 min and concentration 100%. These results concluded that the strain assimilated and sterio inversion of R-3- Chloro-1,2-propanediol via RS 3-chloro-1,2-propanediol. The possibility to produce optically active R-3- Chloro-1,2-propanediol was discussed.