Microbial profiling of South African acid mine water samples using next generation sequencing platform.

Abstract

This study monitored changes in bacterial and fungal structure in a mine water in a monthly basis over 4months. Over the 4-month study period, mine water samples contained more bacteria (91.06%) compared to fungi (8.94%). For bacteria, mine water samples were dominated by Proteobacteria (39.14 to 65.06%) followed by Firmicutes (26.34 to 28.9%) in summer, and Cyanobacteria (27.05%) in winter. In the collected samples, 18% of bacteria could not be assigned to a phylum and remained unclassified suggesting hitherto vast untapped microbial diversity especially during winter. The fungal domain was the sole eukaryotic microorganism found in the mine water samples with unclassified fungi (68.2 to 91%) as the predominant group, followed by Basidiomycota (6.9 to 27.8%). The time of collection, which was linked to the weather, had higher impact on bacterial community than fungal community. The bacterial operational taxonomic units (OTUs) ranged from 865 to 4052 over the 4-month sampling period, while fungal OTUs varied from 73 to 249. The diversity indices suggested that the bacterial community inhabiting the mine water samples were more diverse than the fungal community. The canonical correspondence analysis (CCA) results highlighted that the bacterial community variance had the strongest relationship with water temperature, conductivity, pH, and dissolved oxygen (DO) content, as compared to fungi and water characteristics, had the greatest contribution to both bacterial and fungal community variance. The results provided the relationships between microbial community and environmental variables in the studied mining sites.