Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement.

Emanuel Da Silva Rovai,Marcia Carneiro Valera,Flávia Goulart Da Rosa Cardoso,Felipe De Souza Matos,Frederico Canato Martinho,Warley David Kerbauy,Luciane Dias De Oliveira,Cláudio Antonio Talge Carvalho

doi:10.1590/0103-6440201902471

Emanuel Da Silva Rovai, Marcia Carneiro Valera + Show 6 more

Open Access

https://doi.org/10.1590/0103-6440201902471

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Abstract

This study was carried out to investigate the microbial profile and endotoxin levels of endodontic-periodontal lesions of periodontal origin. Periodontal and endodontic samples were taken from periodontal pockets and necrotic root canals of 10 teeth with endodontic-periodontal lesions. Evidencing of 40 different bacterial species were determined in each endodontic and periodontal sample using the checkerboard DNA-DNA hybridization method and Kinetic chromogenic LAL assay was used for quantification of endotoxins. Fisher's exact test correlated the bacterial species with the endodontic or periodontal microbiota. The endotoxin levels (EU/mL) found in samples of the root canal and periodontal pocket were compared by the Wilcoxon test (p<0.05). Bacteria and LPS units were found in 100% of the endodontic and periodontal samples. The species E. faecium, P. acnes, G. morbillorum, C. sputigena and L. buccalis were strongly correlated with the endodontic microbiota and P. nigrescens with the periodontal microbiota. P. intermedia, P. endodontalis and V. parvula were more prevalent in both endodontic and periodontal microbiots. The endotoxin levels in the periodontal pocket (89600 EU/mL) were significantly higher than in the root canal (2310 EU/mL). It was concluded that the microbiota present in the periodontal and endodontic tissues is similar, with a higher prevalence of species of the orange complex and a higher level of endotoxin in the periodontal pockets.

Highlights

The periodontal and endodontic tissues have embryonic, anatomical and functional interrelationships
Studies that investigated teeth with periodontal and endodontic infection demonstrated that the microorganisms present in the root canals with advanced periodontal disease were similar to those found in the adjacent periodontal pockets [2,4,5,6,7,8]
Prevalence and levels of each bacterial species in endodontic and periodontal samples are presented in Figure 1 and Figure 2, respectively

Summary

Introduction

The periodontal and endodontic tissues have embryonic, anatomical and functional interrelationships. As the tooth and root development, communication pathways are created between the periodontium and pulp, such as dentinal tubules, lateral and accessory canals, and the apical foramen. Through these communications, it is believed that microorganisms and toxins may pervade structures serving as a source for infection in a two-way relationship, resulting in pathological entities known as endodontic-periodontal lesions [1,2]. Endodontic-periodontal lesions are characterized by the association of endodontic and periodontal disease in the same tooth Such infections are typically multispecies and interactions among different microorganisms may contribute to the development of endodontic and periodontal flora [3]. Didilescu et al [9] investigated the association of six bacterial species in endodontic-periodontal lesions and found that the species Fusobacterium nucleatum, Capnocytophaga sputigena and Parviromona micra were more prevalent, suggesting that these species may play a role in the pathogenesis of these lesions

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