Microbial production of short chain diols.

Yudong Jiang,Huibin Zou,Mo Xian,Ning Tian,Wei Liu,Tao Cheng

doi:10.1186/s12934-014-0165-5

Abstract

Short chain diols (propanediols, butanediols, pentanediols) have been widely used in bulk and fine chemical industries as fuels, solvents, polymer monomers and pharmaceutical precursors. The chemical production of short chain diols from fossil resources has been developed and optimized for decades. Consideration of the exhausting fossil resources and the increasing environment issues, the bio-based process to produce short chain diols is attracting interests. Currently, a variety of biotechnologies have been developed for the microbial production of the short chain diols from renewable feed-stocks. In order to efficiently produce bio-diols, the techniques like metabolically engineering the production strains, optimization of the fermentation processes, and integration of a reasonable downstream recovery processes have been thoroughly investigated. In this review, we summarized the recent development in the whole process of bio-diols production including substrate, microorganism, metabolic pathway, fermentation process and downstream process.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-014-0165-5) contains supplementary material, which is available to authorized users.

Highlights

Platform chemicals are the ones that serve as the basic starting materials for producing chemical intermediates, building block compounds, and polymers
Short chain diols, which are exemplified by ethylene glycol, propanediols (PDO), butanediols (BDO), are of great importance in the family of platform chemicals
The distinguished advantage of this strain was the increased extracellular concentration yield of 1,2-PDO because the external discharge capacity of a cell membrane and the processes of releasing bio-metabolites from a cell membrane determined the economic and utility values of bio-metabolites. Another engineered S. cerevisiae strain was constructed by expressing the E. coli mgs and gldA genes, which encode methylglyoxal synthase and glycerol dehydrogenase, respectively [44]

Summary

Introduction

Platform chemicals are the ones that serve as the basic starting materials for producing chemical intermediates, building block compounds, and polymers. An engineered E. coli strain was reported to produce 5.6 g/L 1,2-PDO from glycerol at a yield of 0.21 g/g [41]. The distinguished advantage of this strain was the increased extracellular concentration yield of 1,2-PDO because the external discharge capacity of a cell membrane and the processes of releasing bio-metabolites from a cell membrane determined the economic and utility values of bio-metabolites Another engineered S. cerevisiae strain was constructed by expressing the E. coli mgs and gldA genes, which encode methylglyoxal synthase and glycerol dehydrogenase, respectively [44]. Pure meso-2,3-BDO (purity >99%) was produced at high yield of 0.31 g/g and 0.21 g/g from glucose and crude glycerol feed, respectively, under optimized conditions There is another engineered E. coli capable of producing meso-2,3-BDO from cellodextrin with a yield of 0.84 g/g [19]. A previous communication revealed the production of (2R,4R)-2,4-pentanediol with Candida boidinii RAMIREZ KK912 by the enantio-selective reduction of

Methods

Conclusions

48. Hespell R

54. Paul B

Findings

94. Kelsey DR