Abstract

Aspergillus niger NRRL 567 was cultured on the solid substrate, fruit pomace, in fixed-bed column bioreactors to produce citric acid. The rates of substrate consumption and citric acid production were strongly influenced by (1) the rate of aeration, (2) the fermentation temperature, (3) the initial moisture content of the substrate, and (4) the size of the inoculum. This culture method yielded approximately 130 g of citric acid per kg of apple pomace fermented under optimum conditions.

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