Abstract
Mixed culture ofTrichoderma reeseiandAspergillus nigerwas employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover.
Highlights
As the second largest producer of corn stover in the world,[1] China produces up to 0.3 billion metric tons of corn stover annually
M. isabellina's excellence in single cell oil (SCO) production and its good tolerance towards the inhibitors derived from the pretreatment of lignocelluloses suggest that M. isabellina is a strong candidate for SCO production from cheap agricultural wastes.[5,6]
Filter paper activity (FPA) was measured using the standard method recommended by the International Union of Pure and Applied Chemistry (IUPAC) and expressed in international unit (IU).[16]
Summary
As the second largest producer of corn stover in the world,[1] China produces up to 0.3 billion metric tons of corn stover annually. It is an important resource that could be utilized to produce valuable products in an environment-friendly and sustainable way. This is good for China's environment protection and rural economy. Microbial lipids, regarded as single cell oils (SCO), are more renewable than plant oils or animal fats. They have much shorter production period, don't compete with food production and are much easier to scale up. Many microorganisms can accumulate high content of lipids, among which Mortierella isabellina is able to accumulate more than 80% lipids of its dry cell biomass.[2,3,4] M. isabellina's excellence in SCO production and its good tolerance towards the inhibitors derived from the pretreatment of lignocelluloses suggest that M. isabellina is a strong candidate for SCO production from cheap agricultural wastes.[5,6]
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