Abstract
The gut microbiota is a critical mediator of nutrition and disease risk. Like most complex traits, the microbiome is under genetic regulation and differs between inbred strains of mice. We tested the effect of fecal microbiota transplantation (FMT) on obesity, and plasma glucose. For this study, we collected microbiota from 2 inbred strains of mice which differ in adiposity and glucose tolerance, C57BL/6J and WSB/EiJ. C57BL/6J female mice (n = 18) were first treated with antibiotics for 4 weeks to ablate the microbiota. Following ablation, the mice were transplanted with microbiota from a C57BL/6J or a WSB/EiJ mouse and clinical traits and plasma metabolomic profiles were interrogated at 2- and 4-weeks post-transplantation. Unexpectedly, the mice receiving WSB/EiJ microbiota increased adiposity but decreased plasma glucose. Metabolomic and 16S microbiota profiling indicated broad metabolic changes occurred during and after FMT. Detailed analysis of these interactions demonstrated specific microbiota-host metabolite interactions which may alter disease susceptibility.
Highlights
The gut microbiota is a critical mediator of nutrition and disease risk
We used 18 C57BL/6J female mice who were treated with antibiotics for 4 weeks and received fecal microbiota transplantation (FMT) either from a C57BL/6J or a WSB/EiJ mouse to identify the gut bacteria associated with body composition and metabolic syndrome (MetSyn) risk factors such as plasma glucose and lipid profile
Bacterial genera related to Tyzzerella, ASF356, Acetatifactor, Lachnospiraceae UCG-001, Anaerotruncus, and Marvinbryantia lineage from the donner WSB/EiJ mouse did not get colonized in any recipient mouse
Summary
The gut microbiota is a critical mediator of nutrition and disease risk. Like most complex traits, the microbiome is under genetic regulation and differs between inbred strains of mice. The mice were transplanted with microbiota from a C57BL/6J or a WSB/ EiJ mouse and clinical traits and plasma metabolomic profiles were interrogated at 2- and 4-weeks post-transplantation. Compared to WSB/EiJ mice, C57BL/6J mice have higher fasting blood glucose levels, lower insulin sensitivity, and higher body fat composition[17], and are susceptible to obesity, cardiovascular disease, and MetSyn. C57BL/6J and WSB/EiJ mice have different gut microbiota[18]. We used 18 C57BL/6J female mice who were treated with antibiotics for 4 weeks and received FMT either from a C57BL/6J or a WSB/EiJ mouse to identify the gut bacteria associated with body composition and MetSyn risk factors such as plasma glucose and lipid profile. The association between plasma metabolic profile and phenotypes or gut microbiota were determined by using ANOVA, t-test, principal component (PCA), and Spearman correlation-based statistics
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