Abstract
A Bacillus species was isolated from sewage capable of utilising alkylbenzene sulphonates (ABS) as the sole source of carbon and sulphur. The enzymic mechanism involved in alkyl-side-chain biodegradation of various ABS detergent isomers by the Bacillus species was demonstrated to involve the classical-Β-oxidation equence characteristic of long-chain fatty acid oxidation, by appropriate enzyme inductions. The combined results from both enzyme induction studies and molecular separation of induced enzymes by gel-filtration indicated a single set of enzymes to be responsible for the Β-oxidation of both ABS isomers and long-chain fatty acid isomers in the Bacillus species. The substrate specificity of partially purified enzymes after growth on appropriate substrates confirmed the feasibility of a single Β-oxidation pathway in this microorganism capable of catalising the oxidation of a wide range of different synthetic and naturally occurring chemicals and biochemicals containing alkyl side chains.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.