Microbial inhibition test and optimization of temperature, aeration fermentation of endophytic Fusarium sp LBKURCC 41 from Dahlia tuber (Dahlia variabilis)

Abstract

Link of Video Abstract: https://youtu.be/G8AKREK9E9Q Introduction: Synthetic drugs can cause toxicity. Finding new natural sources of medicinal materials with low toxicity is one of the efforts to reduce it. This was done by using biotechnology from endophytic fungi growing on dahlia tubers (Dahlia variabilis). This study aimed to find the optimum temperature and aeration in the fermentation of endophytic fungi Fusarium sp LBKURCC 41 in producing secondary metabolites. Methods: The method of this research was temperature optimization with temperature variations of 10˚C, 27˚C, 37˚C and 40˚C. For aeration, the variation of oxygen provided was 50 ppb and 100 ppb. Both fermentation environment factors were incubated for 5, 10, 15, 20, and 25 days. The activity test was conducted against pathogenic microbes E. Coli, S. aureus, and C. albicans using the agar diffusion method. Positive controls used ketoconazole for Candida albicans and ciprofloxacin for E. coli and Streptococcus aureus bacteria. Result: The results showed the optimum temperature of the fermentation of endophytic fungi Fusarium sp LBKURCC 41 based on statistical analysis of T-Test for (p < 0.05) is to have the highest inhibition zone diameter value of 10.5 ± 0.62 mm on day 20 against Candida albicans with a positive control of 6.0 ± 0.23 mm. For aeration, the highest inhibition zone value was 12.3 ± 0.12 mm with a positive control of 19.5 ± 0.12 mm. Conclusion: The optimum temperature of the fermentation is to have the highest inhibition zone diameter value of 10.5 ± 0.62 mm. The ability of endophytic fungi to produce secondary metabolite compounds is determined by the temperature and aeration used.