Abstract
Approximately 4600 microorganisms isolated from soil and 110 bacteria from stock cultures were screened for their ability to hydroxylate 3-cyanopyridine (3-CP). Many strains were found to be capable of hydroxylating 3-CP. One of the strains with the highest activity was selected and identified as Comamonas testosteroni (MCI 2848). The product was isolated and identified as 3-cyano-6-hydroxypyridine (6-CHP). We optimized culture and reaction conditions for the production of 6-CHP by resting cells of MCI 2848. The activity was induced by addition of nicotinic acid or pyridine-3-aldoxime to the culture medium. The optimal temperature and pH of the reaction were 28°C and 7.0, respectively. When resting cells was incubated with 3% (w/v) 3-CP, 95% of the 3-CP was converted into 6-CHP within 47 h.
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