Microbial glycerol dialkyl glycerol tetraethers from river water and soil near the Three Gorges Dam on the Yangtze River

Abstract

Suspended particulate matter (SPM) was collected above and below the Three Gorges Dam (TGD) on the Yangtze River in May, July and December 2009, respectively, for a study of the spatial and temporal variation in glycerol dialkyl glycerol tetraethers (GDGTs) in the riverine system. The branched GDGTs (bGDGTs) were > 0.6ng/l and isoprenoid GDGTs (iGDGTs) > 0.3ng/l in most SPM samples in May and July, whereas, iGDGTs were on average about 10× more abundant than bGDGTs in December. The results suggest that the abundance of bGDGTs and iGDGTs in the Yangtze River at the TGD varied seasonally, which might be affected by soil runoff and in situ production. Despite these effects, the TEX86-inferred temperature derived from SPM reflected the measured river surface water temperature in the sampling months, suggesting that planktonic Thaumarchaeota might actively make iGDGTs in the river water near the TGD throughout the year. The bGDGT-derived CBT/MBT temperature from the SPM was not significantly different among the three seasons and the values together averaged 15.2±0.7°C, close to the annual mean air temperature (16.9°C) in the region near the TGD. However, the bGDGT-derived pHCBT values were close to the water pH and slightly higher than the pH of the local soil. These results suggest that the sources of bGDGTs in the river water are complicated and that bGDGTs may not solely derive from the local soil runoff. Nevertheless, our study highlights the potential of using TEX86 and MBT/CBT proxies for a better understanding of the source and mechanism of GDGT production in riverine systems increasingly affected by the construction of dams.