Abstract
Alexei Sorokin and colleagues at the Institut National de la Recherche Agronomique, Jouy en Josas, France, have just reported a low-quality (∼1% error) contiguous sequence of the entire 2.36-Mb genome of Lactococcus lactis IL1403 (http://spock. jouy.inra.fr.) 1 Bolotin A. et al. Low-redundancy sequencing of the entire Lactococcus lactis IL1403 genome. Antonie Van Leeuwenhoek. 1999; 76: 27-76 Crossref PubMed Scopus (158) Google Scholar . L. lactis is an AT-rich Gram-positive microorganism used in the cheese industry, which is also used as a model organism for studying the physiology of lactic acid bacteria. Interestingly, Sorokin et al. supplemented the currently fashionable random shotgun sequencing approach with a range of techniques including multiplex long accurate PCR mapping and primer walking on a variety of templates. Analysis of the genome sequence confirmed the presence of at least four prophages in the chromosome of IL1403, identified ∼40 copies of insertion elements, 15 of which represent an IS1070-like element previously unknown in L. lactis, and identified homologues of all the late competence genes in Bacillus subtilis.
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