Microbial ecology of simultaneous thermophilic microbial leaching and digestion of sewage sludge

Abstract

The microbial population encountered during a simultaneous thermophilic microbial leaching and digestion process at 50 degrees C, based on microbial sulfur oxidation, was investigated. The cell count of the sulfuric acid producer Thiobacillus thermosulfatus increased, followed by a decrease. In the absence of sulfur (control: conventional thermophilic digestion), Thiobacillus thermosulfatus population decreased under the detection limit. Acidophilic and neutrophilic heterotrophic populations increased during the leaching process, and the final acidophilic population count was higher than the neutrophilic population. During the thermophilic digestion (control), the final neutrophilic population count was higher than the acidophilic. Six heterotrophic bacterial strains were isolated and partially characterized. Bacillus was the most predominant genus. The type of bacterial populations in thermophilic microbial leaching and digestion, as well as the thermophilic digestion process (control), were the same, while only the relative concentrations changed. In both processes, the bacterial indicators decreased under the detection limit after 12 h. Mesophilic heterotrophic population was more affected by the thermophilic microbial leaching process than by thermophilic digestion. Sludge mineralization was probably more influenced by the final cell concentration rather than the presence of an individual species or mixed population.