Abstract
A description is given of the biodegradation of diphenylamine (DPA) in a laboratory model sewage system. Ten ..mu..Ci /sup 14/C-DPA and 7.5 mg unlabeled DPA in 5 mL acetone were added to 1.5 L sludge mixed liquor in the incubation flask to produce a solution containing DPA at the 5 ppm level. Extracts were analyzed by gas chromatography/mass spectroscopy. Radioactive extracts were counted with a liquid scintillation spectrometer. DPA was degraded in the model sewage sludge system. Results using 3 different 1.5 L batches of sludge were virtually identical. With our extraction procedure, the average recovery of added radioactivity was 82%. When 300 mL of the sludge mixed liquor was boiled for 5 min before 24 h incubation with 5 ppm DPA, 98% of the radioactivity, as unchanged DPA, was recovered. Ninety-seven percent of the radioactivity, as unchanged DPA, was recovered from 300 mL of nutrient medium alone after 24 h incubation with DPA. These data indicate that the sludge microorganisms alone caused the degradation of DPA. (JMT)
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