Microbial contamination of brand new nickel-titanium endodontic instruments.

Abstract

This study aims to estimate the microbial presence on the surface of different brand new NiTi endodontic instruments for clinical use. Eleven different types of NiTi rotary endodontic instruments, obtained from their fresh opened original packages, were assigned to three different groups, according to packaging type and sterilization and tested for bacterial contamination. Isolated bacteria were identified by using standard microbiological methods and then counted. Differences observed in groups were analyzed statistically by using the one-way analysis of variance (ANOVA) for dependent samples and the Tukey HSD post hoc test. Statistical differences were found between instruments delivered in plastic boxes which bacterial count resulted higher than those obtained from instruments delivered in blisters (p<0.01). Some brand new endodontic instruments showed degrees of bacterial contamination that both quantitatively and qualitatively deserve to be considered in clinical procedures.