Abstract
Problem statement: Soluble sulfate load in effluent is a crucial problem from mining industries. The study involved isolation of efficient Sulfate Reducing Bacterial (SRB) consortium from hot water spring for bioremediatrion of sulfate contaminated waste water. Approach: The enriched bacterial consortium was isolated in medium DSMZ 16695. The sulfate reduction efficiency was measured by turbidometric method. The Km and Rmax value of the consortium was determined. It was immobilized in 10L bioreactor and the sulfate reduction was measured in presence of media and mine effluent. Results: The consortium was found to reduce 2000ppm of sulfate in 36h under optimum condition. The Km of 1.1530 ppm and Rmax value of 0.030h−1 was obtained under optimum conditions of pH-7.5 at 40°C with 2% inoculum. Consortium immobilized under ambient condition in a 10L packed bed reactor yielded about 21-41% reduction of soluble sulfate in synthetic medium prepared using effluent water and tap water respectively. Conclusion: This study reports for the first time the use of efficient SRB consortia from hot water spring for bioremediation of mine effluent.
Highlights
Sulfate concentration is found to rise in natural water due to various anthropogenic activities
The present study reports the sulfate reduction efficiency of the Sulfate Reducing Bacteria (SRB) consortium (G16695), isolated from hot water springs of Taptapani, Orissa in a packed bed bioreactor under ambient condition using both synthetic media and mining effluent water
The vials were incubated at 40°C for 24 h and the growth and Immobilization of Consortium in 10L packed bed bioreactor: In order to be used continuously as a bioremedial package, immobilization was a better choice than suspension culture due to two reasons
Summary
Sulfate concentration is found to rise in natural water due to various anthropogenic activities. It is released into the environment due to various industrial activities (Nasipuri et al, 2010; Liamleam and Annachhatre, 2007) like mining procedure and atmospheric deposition in the form of acid rain (Bull et al, 2001). Increase in sulfate concentration in ground water causes various adverse effects like laxative effect (Zuhair et al, 2008) skin in problem, dehydration, as well as unpleasant taste of water. Increased sulfate concentration causes phosphate mobilization aggravating phosphate eutrophication that can inhibit the growth of different plant species (Geurts et al, 2008). Alternative way of solving the problem is by using Sulfate Reducing Bacteria (SRB) for bioremediation of both sulfate and metals
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