Abstract
The screening of lactic-acid polysaccharide-synthesizing the bacteria, components of kefir grains, has shown that about 60% of isolates can synthesize exopolysaccharides (EPSs). We selected the most actively synthesizing EPSs cultures during development on a medium with lactose or sucrose: Lactococcus lactis and Leuconostoc mesenteroides, respectively. IR-spectroscopy confirmed the identity of the compositions of the synthesized EPSs with polysaccharides of kefir grains. We also determined the parameters for culturing L. mesenteroides on whey with added sucrose that provides a kefiran production level up to 3.4 g/L were developed.
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