Abstract

Compacted Sludge Reducing Barrier (CSRB) was tested to be a feasible barrier for Acid Mine Drainage (AMD) from tailings in case the ground water contamination. Because of its double function (encapsuling and reducing), the microbial community structure diversity in the sludge played a key role. In this paper, we researched the correlation between heavy metals and microbial structure diversity in the dewatered sludge from 4 different sewage treatment process plants in Lanzhou city, a developing area of northwest China, in the colder season. The results indicated that the microbial community structure diversity differed and were unique among the different sewage plants; their correlation with heavy metals was also unique independently.

Highlights

  • The contamination of groundwater by tailings and acidic mine drainage (AMD) is an environmental problem that has long puzzled mining and environmental scientists

  • The sewage sludges were sampled from 4 different waste water treatment plants in Lanzhou city, Gansu province, China, in the beginning of January 2020

  • Some samples were put in the 15 mL sterile centrifuge tube immediately, put in an ice box and transported back to the laboratory, stored in the -70 °C refrigerator waiting for the microbial community structure diversity test

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Summary

Introduction

The contamination of groundwater by tailings and acidic mine drainage (AMD) is an environmental problem that has long puzzled mining and environmental scientists. The problem of safe disposal of dewatered sludge from municipal sewage treatment plants has not been solved effectively for a long time. The effects of different municipal wastewater treatment processes on the microbial community structural diversity in dewatered sludge and the correlation of heavy metal factors in the colder season have been rarely reported. At the beginning of January 2020, dewatered sludges from 4 municipal sewage treatment plants with different locations and different sewage treatment processes in Lanzhou city, China were selected to conduct high-throughput sequencing of microbial community structure diversity research and heavy metal determination, and to conduct horizontal comparison and correlation analysis to obtain more abundant MICROBIAL mechanism data of CSRB

Sampling sites and conditions
Microbial community structure diversity test
Heavy metals content in the sludge
Microbial community structure diversity in the sludge
Correlations between heavy metals and microbial community structure diversity

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