Microbial Community Structure and Diversity in Cellar Water by 16S rRNA High-throughput Sequencing

Abstract

This study was aimed to explore the bacterial diversity of cellar water as well as to study the relationship between the bacterial diversity and environmental factors. The MiSeq high-throughput sequencing was used to analyze and compare the bacterial diversity and community composition of samples from different cellar water samples. Overall 1605 optimized reads were obtained from four samples based on high-throughput sequencing of the V4 region of the 16S rRNA gene. Bacterial species detected in these samples covered 22 phyla,42 classes,71 orders,115 families, 146 genera. Analysis showed that the bacterial diversity was very high in these samples, and there were differences among different samples. The distribution characteristics of the dominant bacteria showed patterns of a large number of rare species and a few common types. Taxonomic assignment analysis indicated that Bacteroidetes,Proteobacteria,Actinobacteria,Verrucomicrobia,OD1 dominated in the Cellar water, and accounted for 87.1% to 94.8% at phylum level. The predominant groups were Actinobacteria,Acidimicrobiia,Cytophagia, Flavobacteriia, Sphingobacteriia,α-Proteobacteria,β-Proteobacteria,γ-Proteobacteria,Opitutae, Verrucomicrobiae,Pedosphaerae and ZB2 at class level. At genus level Rhodobacter,Dechloromonas,Flavobacterium,Acinetobacter,Comamonas,Pseudomonas,Hydrogenophaga,et al were the abundant taxa, which were mainly denitrifying bacteria and heterotrophic nitrification-aerobic denitrification bacteria. The result of RDA suggested that the influences of different environmental factors on different microbes were different. Bacterial community Ⅱ had significant positive correlation with UV254,permanganate index,BOD5,and Bacterial community Ⅲ had significant positive correlation with TN,NO2--N,NO3--N,TP,NH4+-N. This research should deepen the understanding on microbial community in Cellar water, and provide references for the association of bacterial composition and diversity with environmental factors.