Abstract

The existence of nitrite-oxidizing bacteria (NOB) is regarded as one of the main problems in completely autotrophic nitrogen removal over nitrite (CANON) process. In this study, the microbial community structure and distribution of NOB in a lab-scale CANON reactors were investigated at different dissolved oxygen (DO) levels at ambient temperature. Results indicated a distinct community shift of NOB when DO increased from 0.5 mg/L to 1.0 mg/L. Phylogenetic results indicated the predominant NOB were Nitrobacter hamburgensis, Nitrobacter alkalicus and Nitrobacter winogradskyi in the system, all of which coexisted with aerobic ammonium-oxidizing bacteria (AerAOB) and anaerobic ammonium-oxidizing bacteria (AnAOB) without a distinguishable niche on the biofilm.

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