Microbial community diversity and changes associated with a mine drainage gradient at the Dexing copper mine, China

Abstract

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 51:67-76 (2008) - DOI: https://doi.org/10.3354/ame01172 Microbial community diversity and changes associated with a mine drainage gradient at the Dexing copper mine, China Huaqun Yin1,3, Guanzhou Qiu1,3, Liyou Wu2, Ming Xie1,3, Jizhong Zhou2,3, Zhimin Dai1,3, Dianzuo Wang1,3, Laurie Kellogg2, Linhui Cao1,3, Xueduan Liu1,3,* 1School of Minerals Processing and Bioengineering, Central South University, South Lushan Road, Changsha, Hunan 410083, China 2Institute for Environmental Genomics, Department of Botany and Microbiology, Stephenson Research & Technology Center, 101 David L. Boren Boulevard, University of Oklahoma, Norman, Oklahoma 73019, USA 3Key Laboratory of Biometallurgy, Ministry of Education, 25 South Lushan Road, Changsha, Hunan 410083, China *Corresponding author. Email: xueduanliu@mail.csu.edu.cn ABSTRACT: Water samples were collected from 6 sites in the Dexing copper mine, one of the largest open-cast copper mines in China. Each corresponding habitat exhibited distinct geochemical characteristics allowing us to correlate microbial community structure to environmental conditions. We examined the molecular diversity of 16S rRNA genes in water samples from these sites using a PCR-based cloning approach. A total of 68 operational taxonomic units of 16S rRNA genes from 814 screened clones were obtained. The sequenced clones fell into 5 main phylogenetic divisions. The majority (79.1%) of the clones were affiliated with the Gammaproteobacteria (60%), Acidobacteria (8.8%) and Nitrospira (10.3%). However, these sites showed great differences in composition of microbial communities. Moreover, the environmental variables differed greatly across sites; iron, sulfur and calcium concentrations were the variables significantly correlated to the microbial community composition, indicating that Fe2+, S and Ca are possible drivers in shaping microbial community structure. pH was also significantly correlated to microbial community composition at 2 sites. KEY WORDS: 16S rRNA genes · Aquatic microbial communities · Environmental variables Full text in pdf format PreviousNextCite this article as: Yin H, Qiu G, Wu L, Xie M and others (2008) Microbial community diversity and changes associated with a mine drainage gradient at the Dexing copper mine, China. Aquat Microb Ecol 51:67-76. https://doi.org/10.3354/ame01172 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 51, No. 1. Online publication date: April 24, 2008 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2008 Inter-Research.