Microbial communities of a native perennial bunchgrass do not respond consistently across a gradient of land-use intensification

Abstract

To test if native perennial bunchgrasses cultivate the same microbial community composition across a gradient in land-use intensification, soils were sampled in fall, winter and spring in areas under bunchgrasses (‘plant’) and in bare soils (‘removal’) in which plots were cleared of living plants adjacent to native perennial bunchgrasses ( Nassella pulchra). The gradient in land-use intensification was represented by a relict perennial grassland, a restored perennial grassland, and a perennial grass agriculture site on the same soil type. An exotic annual grassland site was also included because perennial bunchgrasses often exist within a matrix of annual grasses in California. Differences in soil resource pools between ‘plant’ and ‘removal’ soils were observed mainly in the relict perennial grassland and perennial grass agriculture site. Seasonal responses occurred in all sites. Microbial biomass carbon (C) and dissolved organic C were greater under perennial bunchgrasses in the relict perennial grassland and perennial grass agriculture site when comparing treatment means of ‘plant’ vs. ‘removal’ soil. In general, soil moisture, microbial respiration, and nitrate decreased from fall to spring in ‘plant’ and ‘removal’ soils, while soil ammonium and net mineralizable nitrogen (N) increased only in ‘plant’ soils. A canonical correspondence analysis (CCA) of phospholipid fatty acid (PLFA) profiles from all sites showed that land-use history limits the similarity of microbial community composition as do soil C and N dynamics among sites. When PLFA profiles from individual sites were analyzed by CCA, different microbial PLFA markers were associated with N. pulchra in each site, indicating that the same plant species does not retain a unique microbial fingerprint across the gradient of land-use intensification.