Microbial cell lysis and nucleic acid extraction via nanofluidic channel

Krishna Kant,Dusan Losic,Joe G Shapter,Mason Erkelens,Jeongha Yoo,Craig Priest,Steven Amos

doi:10.1039/c5ra01336d

Abstract

This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction.

Highlights

Nano uidic techniques enable extreme miniaturization of devices and enhanced analytical performance
This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction
Once we have the extended microchannel using FIB, the distance between the microchannels is much smaller and we can mill a nanochannel with low energy (0.17 nA) ion beam in a very short time (2–3 min depending on the Cr layer thickness)

Summary

Introduction

Nano uidic techniques enable extreme miniaturization of devices and enhanced analytical performance. This scheme shows the ow of algae cells used as a model into the microchannels and diffusion of NaCl through the nanochannel for cell lysis. Cell injection and lysis into micro uidic channel the microchannel where the cells were trapped. 1 Â 105 cells per ml diluted with deionized (DI) water in Fig. 3(i) was injected into the R1 port of the micro uidic channel by using a syringe pump (Chemyx, Fusion-200) with ow rate of 3 ml minÀ1. The quantity of DNA was displayed and purities of nucleic acids were monitored by 260 nm/280 nm absorbance ratio, which gives the quality of the DNA extraction.[25]

Result and discussion

Conclusions