Abstract
The reliability of three methods (microbial C and mineral-N flush by fumigation-incubation, and ATP) for measuring soil microbial biomass was assessed on two silt-loam soils of different P fertility status under grazed perennial pastures. The mineral-N flush and ATP methods provided a reasonably reliable index of microbial biomass, but the fumigation-incubation procedure for CO2-C flush, using preincubated samples and an unfumigated 0–10 day control, was inappropriate for these soils. The numbers of bacteria (direct microscopy) and the percentage metabolically active were also measured.
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