Microbial and histological analysis of rat model of oral candidiasis

Abstract

Objective: C. albicans infection is the most common opportunistic infection in humans, and the prevalence of this infection has been increasing recently. Exploratory research on materials that have antifungal potential is urgently needed. One of the stages in the study is the use of experimental animal models. By various extrapolations, we designed models of oral candidiasis for men and women. This study aims to evaluate the infection after the treatment we provide, both microbiologically and histologically. Material and Methods: Our male and female rats lowered their immune systems with the corticosteroid methylprednisolone. The rats' drinking water was mixed with antibiotics to disrupt the balance of the oral cavity's normal flora. To facilitate C.albicans exposure and avoid loss of C.albicans, rats were previously injected with chlorpromazine HCL. Results: The microbiological evaluation showed that in male and female rat models, the number of fungal colonies on oral mucosal swab cultures was significantly higher at five and eight days after exposure to putrefactive control mice. Histological evaluation showed that in model rats, the number of hyphae that penetrated the dorsum of the tongue was higher than in control rats five days after exposure. Conclusion: In conclusion, the method we used is proven to produce oral candidiasis model rats, both male, and female