Microbial Alpha-Amylase Production: Progress, Challenges and Perspectives.

Babak Elyasi Far,Azita Dilmaghani,Ahmad Yari Khosroshahi,Yassin Ahmadi

doi:10.34172/apb.2020.043

Babak Elyasi Far, Azita Dilmaghani + Show 2 more

Open Access

https://doi.org/10.34172/apb.2020.043

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Abstract

Alpha-amylase reputes for starch modification by breaking of 1-4 glycosidic bands and is widely applied in different industrial sectors. Microorganisms express unique alpha-amylases with thermostable and halotolerant characteristics dependent on the microorganism’s intrinsic features. Likewise, genetic engineering methods are applied to produce enzymes with higher stability in contrast to wild types. As there are widespread application of α-amylase in industry, optimization methods like RSM are used to improve the production of the enzyme ex vivo. This study aimed to review the latest researches on the production improvement and stability of α-amylase.

Highlights

Compared to the chemical methods that need harsh conditions such as high pressure and temperature, using of microorganism is considered in many purposes including heavy metal absorption,[1] gene engeering,[2] digestion,[3] production of novel anti-microbs[4] and particullary for producing of industrial enzymes.[5,6] Demand on the high-quality productions leads to development of novel methods to improve industrial products such as protease and amylase that are frequently applied in industry and medical science.There have been identified three types of amylase including α-amylase, β-amylase, and γ-amylase
Alpha-amylase reputes for starch modification by breaking of 1-4 glycosidic bands and is widely applied in different industrial sectors
This study aimed to review the latest researches on the production improvement and stability of α-amylase

Summary

Introduction

Compared to the chemical methods that need harsh conditions such as high pressure and temperature, using of microorganism is considered in many purposes including heavy metal absorption,[1] gene engeering,[2] digestion,[3] production of novel anti-microbs[4] and particullary for producing of industrial enzymes.[5,6] Demand on the high-quality productions leads to development of novel methods to improve industrial products such as protease and amylase that are frequently applied in industry and medical science.There have been identified three types of amylase including α-amylase, β-amylase, and γ-amylase. Alphaamylase is an industrial enzyme (EC 3.2.1.1.), which cleaves internal alpha 1-4 glycosidic bands of starch and other polysaccharides to produce several products such as glucose and maltose.[7,8] It belongs to the family of GH13 (most of them), GH57, GH119, and GH1269 and is one of the most widely used commercial enzymes.[10]. Regarding the outstanding application of α-amylase, there is an urgent need to develop the costeffective techniques to produce stable and efficient alphaamylase.[11] Here we aim to present different methods of the amylase production and how it is possible to improve the efficiency of alpha-amylase

Objectives

Findings

Conclusion