Abstract
Steroid 9alpha-hydroxylase is a key enzyme system in steroid nucleus degradation in company with Delta(1)-dehydrogenase. To examine 9alpha-hydroxylase activity during microbial transformation of steroids, 9(11)-dehydro-17alpha-methyl-testosterone was adopted as a stable substrate for preventing the rupture of steroid nucleus. UsingNocardia restrictus ATCC 14887 capable of introducing a 9alpha-hydroxyl group into steroids, 9alpha, 11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one and 9alpha, 11alpha-oxido-17beta-hydroxy-17alpha-methyl-1, 4-androstadiene-3-one were obtained. These microbiologically transformed products could be used as reference compounds in the enzyme assay.
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