Abstract
To study the biological role of the tynA gene product of Escherichia coli, a primary amine oxidase (ECAO, E.C. 1.4.3.21), the tynA gene was genetically silenced by conjugation with a kanamycin resistance cassette. We used a microarray method to compare the mRNA expression in the modified strain (ΔtynA) to that in the wild type (wt) strain at the time of induction of ECAO expression (0 h) as well as 1 h and 4 h after the induction. These data in brief describe the different experimental conditions, sample preparation, data collection and analysis of the conducted microarray experiment. The differential expression of genes in the studied strains 1 h after the induction of ECAO expression is described. The microarray data have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE65385.
Highlights
To study the biological role of the tynA gene product of Escherichia coli, a primary amine oxidase (ECAO, E.C. 1.4.3.21), the tynA gene was genetically silenced by conjugation with a kanamycin resistance cassette
We constructed a genetically modified E. coli K-12 strain unable to express ECAO (ΔtynA, [1]). Both wild type and ΔtynA strains were cultured in conditions, which induce the expression of ECAO, to be able to compare the expression profiles of ΔtynA and wt bacteria
Biotinylated cDNA fragments, 2.4 μg for each experimental condition, were hybridized to GeneChip E. coli Genome 2.0 Array at 45 °C for 16 h according to the GeneChip Expression Analysis Technical Manual [2]
Summary
Detailed information about the generation of the modified ΔtynA strain is published [1]. We introduced a kanamycin cassette into the tynA gene by homologous recombination utilizing a bacterial conjugation to silence the gene
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