Abstract

The early stages of drug discovery heavily rely on high-throughput screening (HTS) of compound libraries to identify effective lead compounds. Biochemical and cell-based assays have been commonly performed with the assistance of robotic liquid dispensing systems to rapidly test and identify the potential efficacy and toxicity of drug candidates [1, 2]. In an effort to save costs and reduce expensive resources such as primary human cells and reagents, pharmaceutical industries have focused on miniaturizing HTS assays by using higher density well plates including 384- and 1536-well plates, leading to a reduction in reagent volumes and an increase the speed of the liquid dispensing [1, 3].

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