Abstract
The Rag2 knockout (KO) mouse is one of the most popular immune compromised animal models used in biomedical research. The immune compromised state concurrently alters many signalling pathways and molecules, including miRNAs and mRNA transcripts that are involved in important biological processes. In addition, miRNAs and transcripts are interdependent, often forming a feedback loop; dysregulation in one might alter the expression of the other, and both participate in many physiological processes including immune regulation. Here, we describe a comprehensive dataset containing alterations in the expression of both miRNAs and mRNAs in Rag2 KO mice compared to their wild type counterparts. The miRNA and mRNA expression profiles were generated from total RNA using a miRNA expression microarray or a BeadChip microarray, respectively. Hence, this dataset will provide the groundwork for a comparative study of the miRNAs and mRNAs that are dysregulated in Rag2 KO mice. It is hoped that the data will illuminate how miRNAs mediate immune regulation, as well as the interaction between miRNAs and mRNAs in Rag2 KO mice.
Highlights
Due to the leaky characteristics of many severe combined immune deficiency (SCID) animals[1], alternative mice strains with more stable defective immune system have been developed, including the Rag[1] and Rag[2] knockout (KO) mice[2,3]
The deposited dataset offers an opportunity to investigate the global changes in miRNAs and mRNA expression in the Rag[2] KO mice
MicroRNAs are non-coding small RNA molecules that posttranscriptionally regulate the expression of approximately one third of human genes either by inducing mRNA degradation or by inhibiting translation[4,5]
Summary
Due to the leaky characteristics (especially at older ages) of many severe combined immune deficiency (SCID) animals[1], alternative mice strains with more stable defective immune system have been developed, including the Rag[1] and Rag[2] knockout (KO) mice[2,3]. The Rag[2] and Rag[1] proteins form a complex, which is able to create double-strand breaks by cleaving DNA at conserved recombination signal sequences and contribute to B- and T-cell development. Due to this essential role, depletion of the Rag[2] gene successfully impairs the development of both B- and T-cells[3]. Evidence has shown that miRNAs could alter genes expression and signalling in the immune system, and regulate different biological and cellular processes[6,7,8,9,10]. The recent mouse genome reference is GRCm38 having higher number of genes (around 45,300 genes), but the data could be reanalysed using miRNA Expression Microarray Analysis
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