Abstract
Accumulating evidence has revealed that aberrant Circular RNAs (circRNAs) expression plays important roles in carcinogenesis and tumor progression. However, their role in non-small cell lung cancer (NSCLC) remains unclear. In this study, we first used circRNA microarrays to screen for tumour-specific circRNA candidates in between NSCLC (n = 3) and adjacent lung (n = 3) tissue. Among the circRNA expression profile, two circRNAs (hsa_circ_0014130 and hsa_circ_0016760) were selected for validation in ten pairs of NSCLC and adjacent non-cancerous tissues by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only hsa_circ_0014130 exhibited significantly overexpressed in NSCLC tissues (P < 0.001), which were further confirmed in another 36 matched tissue samples using qRT-PCR. Hsa_circ_0014130 expression significantly correlated with TNM stage (P = 0.001) and lymphatic metastasis (P = 0.004). The area under the receiver operating characteristic curve was 0.878 (95% confidence interval = 0.804–0.951; P < 0.001), which showed good diagnostic potential. Bioinformatics platforms predicted that hsa_circ_0014130 might interact with five miRNAs and their corresponding mRNAs. Gene oncology analysis and pathway analysis revealed that hsa_circ_0014130 could participate in NSCLC development. In summary, our findings indicated that hsa_circ_0014130 could be used as a potential NSCLC biomarker and might be closely related to the carcinogenesis of NSCLC.
Highlights
Lung cancer is the leading cause of cancer death among males in both more and less developed countries, and has surpassed breast cancer as the leading cause of cancer death among females in more developed countries[1]
Total RNA was extracted from each sample, and the results of RNA quality and RNA integrity were displayed in Supplementary Figure 1
Using high-throughput human circRNA microarray, we assessed the differences of circRNA expression profiles between non-small cell lung cancer (NSCLC) tissues and paired adjacent non-cancerous tissues from 3 patients
Summary
Lung cancer is the leading cause of cancer death among males in both more and less developed countries, and has surpassed breast cancer as the leading cause of cancer death among females in more developed countries[1]. Hsa_circ_0013958 has been indicated to be used as a potential non-invasive biomarker for the early detection and screening of lung adenocarcinoma[19]. Despite this potential link with circRNAs, the global circRNA expression profile in NSCLC has not been fully uncovered. A bioinformatics analysis was used to predict the miRNA binding sites and the related mRNAs of hsa_circ_0014130 These results indicate that the upregulated expression of hsa_circ_0014130 significantly associates with some clinicopathological factors of NSCLC patients, and may serve as a novel potential tumor marker and therapeutic target for NSCLC
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