Abstract

Shreffler WG, Beyer K, Chu TH, Burks AW, Sampson HA. J Allergy Clin Immunol . 2004;113:776–782 To develop a peptide microarray-based immunoassay to map IgE-binding segments (epitopes) of peanut allergens by using microliter quantities of serum. Sera from 77 peanut-allergic patients and 15 non–peanut-allergic control patients were analyzed. A set of 213 overlapping 20-residue peptides was synthesized corresponding to the primary sequences of the major peanut allergens, Ara h1, …

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