Abstract
Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.
Highlights
The precise formation of chromatin by DNA and histones is essential for almost all types of biological phenomena including cell proliferation, differentiation, and migration
H2B monoubiquitination is a key histone modification that plays a significant role in various biological processes including stem cell differentiation, DNA damage response, and chromatin organization
Using this direct interference method, we revealed that overexpression of the H2BK120R mutant construct in human cell lines efficiently suppresses endogenous H2B monoubiquitination levels (Fig 1)
Summary
The precise formation of chromatin by DNA and histones is essential for almost all types of biological phenomena including cell proliferation, differentiation, and migration. A nucleosome is a repeating unit of chromatin that contains approximately 147 base pairs of DNA and core histones (H3, H4, H2A, and H2B). DNA is wrapped around octamers formed by these core histones [1,2,3,4]. It has been well established that histones can be modified by distinct and specific enzymes causing various modifications.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
