Microalgae Enhance the Resistance of Pond-Dwelling Ammonia-Oxidizing Bacteria to Light Irradiation

Abstract

Pond aquaculture is an important aquacultural model worldwide in which ammonia-oxidizing bacteria (AOB) are crucial for the removal of ammonia from water. The influence of light irradiation on AOB in an aquaculture pond was studied using artificial simulation wastewater under dark/light cycles of 24 h/0 h (L0), 12 h/12 h (L12), and 0 h/24 h (L24). The ammonia oxidation rates (AORs) in groups L0, L12, and L24 were 9.88 ± 0.19 mg h−1, 6.01 ± 0.32 mg h−1, and 1.85 ± 0.09 mg h−1, respectively. Long-term exposure to light had a serious impact on the AOR and decreased the abundance of Nitrosomonas spp. and their ammonia monooxygenase genes. To determine the protective effect of microalgae on AOB, different doses of freeze-dried Chlorella spp. powder were added to the nitrifying bacteria community. The photoinhibition rate of chlorophyll a (Chla) in the groups with 300 and 1300 µg L−1 of added Chlorella were 32.85% and 28.77%, respectively, while the Chla in the 2200 µg L−1 Chlorella-added group was only 0.01%, with no significant differences (p > 0.05) in AOR between the dark/light treatment subgroups. Fluorescence in situ hybridization showed that AOB, nitrite-oxidizing bacteria, and algae coexist and grow together without free AOB in the nitrifying bacterial community. It was suggested that microalgae enhance the resistance of AOB to light irradiation in a pond through the shading effect provided by algal chlorophyll and the close symbiotic relationship between microalgae and AOB.