Microbial Cell Factories | VOL. 21

Microaerobic metabolism of lactate and propionate enhances vitamin B12 production in Propionibacterium freudenreichii

Publication Date Oct 28, 2022


BackgroundPropionibacterium freudenreichii is used in biotechnological applications to produce vitamin B12. Although cultured mainly in anaerobic conditions, microaerobic conditions can greatly enhance biomass formation in P. freudenreichii. Since B12 yields may be coupled to biomass formation, microaerobic conditions show great potential for increasing B12 yields in P. freudenreichii.ResultsHere we show biomass formation increases 2.7 times for P. freudenreichii grown in microaerobic conditions on lactate versus anaerobic conditions (1.87 g/L vs 0.70 g/L). Consumption of lactate in microaerobic conditions resulted first in production of pyruvate, propionate and acetate. When lactate was depleted, pyruvate and propionate were oxidised with a concomitant sixfold increase in the B12 titer compared to anaerobic conditions, showing potential for propionate and pyruvate as carbon sources for B12 production. Consequently, a fed-batch reactor with anaerobically precultured lactate-grown cells was fed propionate in microaerobic conditions resulting in biomass increase and production of B12. Vitamin yields increased from 0.3 mu g B12 per mmol lactate in anaerobic conditions to 2.4 mu g B12 per mmol lactate and 8.4 mu g B12 per mmol propionate in microaerobic conditions. Yield per cell dry weight (CDW) increased from 41 mu g per g CDW in anaerobic conditions on lactate to 92 mu g per g CDW on lactate and 184 mu g per g CDW on propionate in microaerobic conditions.ConclusionsHere we have shown both B12 yield per substrate and per CDW w...


Production Of B12 Microaerobic Conditions Cell Dry Weight Mmol Lactate Anaerobic Conditions Biomass Formation Consumption Of Lactate Production Of Pyruvate Biomass Increase Vitamin B12

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