Abstract
A procedure was established for micro-two-dimensional gel electrophoresis with agarose gels in the first dimension. Agarose (Agarose IEF, Pharmacia) was previously found to be suitable for the analysis of high molecular weight proteins with a wide range of isoelectric points, but rather difficult to be handled for micro-scale electrophoresis. However, addition of 1M thiourea in combination with 5M urea to the medium for agarose gel made it possible to be used with ease on micro-scale as well as on macro-scale. Since thiourea is a potent protein solubilizing reagent, almost all amounts of proteins including structural proteins were presented on the respective positions on two-dimensional gels. This method is applicable to other experiments with high molecular weight proteins to be analyzed in a wide range of isoelectric points.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.