Micro Salting-Out Assisted Matrix Solid-Phase Dispersion: A Simple and Fast Sample Preparation Method for the Analysis of Bisphenol Contaminants in Bee Pollen.

Jianing Zhang,Wenbin Chen,Xijuan Tu,Yunmin Tao,Chunping Du,Fengjie Yu,Wenchao Yang

doi:10.3390/molecules26082350

Jianing Zhang, Wenbin Chen + Show 5 more

Open Access

https://doi.org/10.3390/molecules26082350

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Abstract

In the present work, a novel sample preparation method, micro salting-out assisted matrix solid-phase dispersion (μ-SOA-MSPD), was developed for the determination of bisphenol A (BPA) and bisphenol B (BPB) contaminants in bee pollen. The proposed method was designed to combine two classical sample preparation methodologies, matrix solid-phase dispersion (MSPD) and homogenous liquid-liquid extraction (HLLE), to simplify and speed-up the preparation process. Parameters of μ-SOA-MSPD were systematically investigated, and results indicated the significant effect of salt and ACN-H2O extractant on the signal response of analytes. In addition, excellent clean-up ability in removing matrix components was observed when primary secondary amine (PSA) sorbent was introduced into the blending operation. The developed method was fully validated, and the limits of detection for BPA and BPB were 20 μg/kg and 30 μg/kg, respectively. Average recoveries and precisions were ranged from 83.03% to 94.64% and 1.76% to 5.45%, respectively. This is the first report on the analysis of bisphenol contaminants in bee pollen sample, and also on the combination of MSPD and HLLE. The present method might provide a new strategy for simple and fast sample preparation of solid and semi-solid samples.

Highlights

In the past decade, matrix solid-phase dispersion (MSPD) has achieved great progress in the sample preparation of complex samples [1,2,3,4]
The sample is blended with salt and μ-SOA-MSPD
According to the principle of MSPD, this step is designed for the disruption of Firstly, the sample is blended with salt and sorbent by way of the classical MSPD methodsolid sample by using the shearing and grinding force in the blending operation

Summary

Introduction

Matrix solid-phase dispersion (MSPD) has achieved great progress in the sample preparation of complex samples [1,2,3,4]. Vortex and homogenization have been reported to replace the column elution and blending in the classical MSPD, respectively, to simplify the procedure of preparation [9,10,11,12,13,14] Another method for the replacement of column was the magnetically assisted MSPD, in which magnetic ion liquid [15] or particles [16] were used as dispersants. Micro/mini-MSPD, which reduced the sample amount in the protocol, was a good choice for reducing the consumption of sample and material [17,18,19,20,21,22,23,24] and improving the greenness of MSPD [25] These improved methods have promoted the development of sample preparation technology for solid and semi-solid sample

Methods

Results

Conclusion