Abstract
Micro-RNA has a very important role in gene regulation. The stem gene Oct4 is related with the growth, mobility, and infiltration of skin cancer cells. Studying the regulatory mechanism of micro-RNAs of Oct4 in skin cancer cells is of important clinical significance. Oct4 gene was analyzed using bioinformatics methods to find mir-RNA with regulatory functions. mir-RNA high-expression vector and suppression vector with pcDNA3.1-EGFP was transfected to skin cancer cell line HS-4 followed analysis of Oct4 expression 24 h and 48 h after transfection and transwell in-vitro cell invasion assay. Bioinformatics showed that mir-335 has relationship with the Oct4 gene. pcDNA3.1-EGFP-335-up and pcDNA3.1-EGFP-335-down were successfully constructed. 24 and 48 hours after transfection, the Oct4 expression in the high-expression group was gradually and significantly decreased (P < 0.05). Meanwhile, the cell migration and infiltration capacity was decreased significantly and showed time dependence with significant differences between groups (P < 0.05). mir-335 expression in suppression group was reduced without change of Oct4 (P > 0.05). Increased mir-335 can decrease the performance of Oct4 in skin cancer cells and inhibit the infiltration ability of cells without affecting cell infiltration capability.
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