Micro‐RNA isolated from liquid biopsies serve as novel molecular biomarkers for congestive heart failure in dogs

Abstract

ObjectiveTo characterize expression profiles of circulating microRNAs via miRNA isolation from plasma (Qiagen) for dogs with congestive heart failure (CHF) secondary to myxomatous mitral valve degeneration (MMVD).Materials and Methods9 healthy small breed client‐owned dogs and 9 small breed client‐owned dogs (ages 9 and up) with CHF secondary to MMVD. Blood samples were collected from both healthy and affected dogs. Isolated microRNAs from plasma were subjected to quantitative reverse transcription PCR (qRT‐PCR) used to examine expression profiles of differentially expressed circulating microRNAs of dogs with CHF.ResultsResults of qRT‐PCR assays confirmed up‐regulation of several microRNAs as well as downregulation of multiple microRNAs. Results of qRT‐PCR assays were highly correlated with NGS data from a prior study done and supported our results.ConclusionThese results suggested that miRNAs serve as novel biomarkers for congestive heart failure (CHF) in small breed canines.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.