Abstract
Breast cancer is the most common cause of cancer-related death among women in the whole world. MiR- 34a and let-7a are well known tumor suppressors that participate in the regulation of apoptosis, invasion and other cellular functions. In this study, expression of miR-34a, let-7a and apoptosis pathway genes such as Bcl-2, Caspase-3 and P53 were evaluated using quantitative real-time PCR in 45 paired samples of normal margin and tumor tissue collected from breast cancer patient at advanced stage (3-4). MiR-34a, let-7a, caspase-3 and P53 expression are reduced and Bcl-2 expression is increased within tumoral tissues in comparison with normal margin tissues. P53 expression directly or indirectly was correlated with miR-34a, let-7a, Bcl-2 and caspase-3 expression. In This study we found that MiR-34a and let-7a expression are reduced in the tumoral tissues. Down- regulation of these two molecules correlated with expression of genes associated with apoptosis. These results suggest that due to the correlation of miR-34a and let-7a with apoptotic and anti-apoptotic pathways these molecules could participate as regulators in advanced clinical stages of breast cancer and should be considered as markers for diagnosis, prognostic assessment and targeted therapy.
Highlights
Breast cancer is the most common cause of cancerrelated death among women worldwide, and is the molecular and clinically heterogeneous (Ong et al, 2015)
Expression of miR-34a, let-7a and apoptosis pathway genes such as Bcl-2, Caspase-3 and P53 were evaluated using quantitative real-time PCR in 45 paired samples of normal margin and tumor tissue collected from breast cancer patient at advanced stage (3-4)
Bcl2 expression was mainly observed in the advanced front of the tumoral tissues, interestingly caspase3, p53 expression was decreased in tumoral tissues compared to normal marginal tumoral tissue, suggesting its effect on tumor progression
Summary
Breast cancer is the most common cause of cancerrelated death among women worldwide, and is the molecular and clinically heterogeneous (Ong et al, 2015). Small RNA regulator in the field of cancer research was noted. MicroRNAs are small noncoding RNAs which have 19-22 nucleotides (Mansoori et al, 2014) and participitated in the stability or translation of mRNA in post-transcriptional regulation of gene expression in multicellular organisms (Davis et al, 2006; Costa and Pedroso de Lima, 2013). The altering expression of MiRNAs profile have been identified as modulators of proliferation, apoptosis and resistance treatment in breast cancer (Iorio et al., 2008). The dysregulation of specific miRNAs was correlated with breast cancer (Hannafon et al, 2011). The concept of specific miRNA expression pattern and the expression of related gene with this, insight into choose a best targeted therapy for these patients (Yao et al, 2015)
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